Mycology VIVA Question and Answer

Last Updated on November 9, 2020 by Sagar Aryal

Mycology VIVA Question and Answer
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1) What is Mycology?

  • It is the study of fungi which includes yeasts and molds.

2) Fungi is prokaryotic or eukaryotic? 

  • Eukaryotic

3) Example of a unicellular eukaryotic organism?

  • Yeast

4) What are saprophytes?

  • Those organisms which depend on( live on) dead and decaying organism are saprophytes. Example: Fungi

5) What is the difference in the cell membrane of fungi and bacteria?

  • Sterol is present in the cell membrane of fungi i.e ergosterol
  • In bacteria, there is an absence of sterol except in Mycoplasma

6) What is the cell wall of fungi made up of?

  • Chitin
  • Glucans
  • Mannans
  • Peptidoglycan is not present in it.

7) Name symbiotic fungi and it’s use.

  • Mycorrhizae
  • Helps root in absorbing minerals and water from the soil

8) Give examples of yeast:

  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe
  • Candida albicans
  • Cryptococcus neoformans

9) Give examples of mold:

  • Penicillium
  • Rhizopus
  • Aspergillus
  • Fusarium
  • Mucor
  • Cladosporium
  • Trichophyton
  • Trichoderma 

10) What do you mean by Dimorphic fungi?

  • Having two forms i.e. Yeast and Mold
  • Temperature-dependent pathogenic fungi are yeast-like at 35 °C – 37 °C and mold like at 25 °C- 30 °C.

11) Give examples of Dimorphic fungi?

  • Histoplasma capsulatum
  • Candida albicans
  • Blastomyces dermatitidis
  • Coccidioides immitis
  • Paracoccidioides brasiliensis
  • Penicillium marneffei
  • Sporothrix schenckii

12) What do you mean by polymorphic fungi?

  • Fungi that have more than one independent form or spore stage in their lifecycle are called polymorphic fungi.

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13) Name one of the enzymes present in fungi and it’s use.

  • Cellulase
  • Animals cannot digest the hard part of the plant so fungi as a primary decomposer use cellulase enzyme for the decomposition.

14) What is Thallus?

  • The thallus is the body or vegetative structure that is present in fungus.
  • Its plural is Thalli

15 ) What are hyphae?

  • Long, filamentous, and branching structures present in fungi are called hyphae.
  • It’s singular is hypha.

16) What are mycelium?

  • The filamentous mass of hypha are called mycelium.
  • They are visible to the naked eye.

17) What are septa?

  • It is the cross wall present in hyphae.
  • It divides the hypha into different distinct parts, uninucleate (one-nucleus ) cell-like units.

18) What are septate hyphae? 

  • Hyphae which contains septa in it.

19) What are coenocytic hyphae?

  • Hyphae which doesn’t contain septa are called coenocytic hyphae.
  • Also called Aseptate or Non-septate hyphae.

20) What is hyaline?

  • It is non-pigmented hyphae.

21) What is dematiaceous?

  • It is darkly pigmented hyphae.

22) What is conidia?

  • Conidia mean asexual spores.

23) What are the types of asexual spores?

  • Arthroconidia or arthrospores
  • Chlamydospores
  • Sporangiospores
  • Conidiospores
  • Blastospores

24) What are the types of sexual spores?

  • Zygospore
  • Ascospore
  • Basidiospore

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25) What are teleomorphs?

  • Teleomorphs are fungi that produce both sexual and asexual spores.

26) What are anamorphs? 

  • Asexual form in fungi of phyla Ascomycota and Basidiomycota are anamorphs.

27) What are synanomorphs? 

  • Fungi that have a different asexual forms of the same fungus are called synanomorphs.

28) What are arthroconidia or arthrospores?

  • The spores ( cells) formed by the fragmentation of the hypha are called arthroconidia or arthrospores.
  • Fragmentation occurs through the splitting of the cell wall or septum.

29) What are chlamydospores?

  • Before the separation, if the cells are surrounded by a thick wall, they are called chlamydospores.

30) What are sporangiospores?

  • If the spores develop within a sac at a hyphal tip, they are called sporangiospores.
  • The saclike structure is sporangium ( plural: sporangia )

31) What are conidiospores?

  • If the spores are produced at the tips or sides of the hypha but are not enclosed in a sac, they are called conidiospores.

32) What are blastospores? 

  • The spores which are produced from a vegetative mother cell by budding are called blastospores.

33) General feature of Colonies of yeast :

  • Moist
  • Creamy
  • Opaque or pasty colonies

34) General feature of filamentous fungi or mold :

  • Fluffy
  • Cottony
  • Powdery colonies

35) What is the fungal infection termed as? 

  • Mycosis ( plural mycoses )

36) Medical classification of fungi : 

  • Superficial and Cutaneous mycoses: fungal infection of the outermost layers of skin and hair
  • Subcutaneous mycoses: fungal infection beneath the skin i.e  dermis, subcutaneous tissue
  • Systemic mycoses: fungal infection deep within the body
  • Opportunistic mycoses: a fungal infection caused by the opportunistic pathogen

37) What is otomycosis?

  • fungal infection of the ear

38) What is osteomycosis? 

  • fungal infection of the bone

39) Examples of Superficial and Cutaneous mycoses :

Disease  Associated fungi  
Pityriasis Versicolor ( dandruff and follicular pityriasis )Malassezia furfur
Tinea pedis ( Athlete’s foot)/ Tinea cruris ( ringworm )Trichophyton rubrum
Piedra (trichosporosis) (black and white )Piedraia hortae and Trichosporon beigelii

40) Examples of Sub-cutaneous mycoses : 

Infection Associated fungi
SporotrichosisSporothrix schenckii
ChromoblastomycosisPhialophora spp. , Cladosporium spp.
Mycotic mycetomaPseudellescheria spp. , Madurella spp. , Exophila spp. , Cladosporium spp.

41) Examples of Systemic mycoses : 

InfectionAssociated fungi
CryptococcosisCryptococcus neoformans
HistoplasmosisHistoplasma capsulatum
BlastomycosisBlastomyces dermatitidis
ParacoccidioidomycosisParacoccidioides brasiliensis
CoccidioidomycosisCoccidiodes immitis

42) Examples of Opportunistic mycoses : 

InfectionAssociated fungi
AspergillosisAspergillus fumigates
CandidiasisCandida albicans
GeotrichosisGeotrichum spp.
PenicillosisPenicillium marneffei
PneumicystosisPneumocystis carinii

43) What are the commonly used laboratory media for the isolation of fungi from clinical specimen :

Primary recovery media : 

  • Brain-heart infusion agar
  • Mycosel agar

Differential test media

  • Cornmeal agar with Tween 80 and trypan blue
  • Czapek’s agar
  • Niger seed agar
  • Nitrate reduction medium
  • Yeast fermentation broth
  • Potato Dextrose Agar (PDA)

Reference and Sources

  • Tille PM (2014) , Bailey and Scott’s Diagnostic Microbiology, 13th edition, Mosby Elsevier
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