Culture media used in microbiology

Culture media used in microbiology

Culture media used in microbiology

Image Source: Sigma-Aldrich

  1. Nutrient agar(1% to 2%) –> Basal media in microbiology, Supports the growth of all non-fastidious organisms
  2. MacConkey Agar—> Differential media for Enterobacteriaceae ( i.e. Lactose fermenting and non-lactose fermenting)
  3. Mannitol Salt Agar–> Selective and indicator media for S. aureus
  4. Triple Sugar Iron(TSI) medium–> Differentiation of various members of Enterobacteriaceae
  5. Robertson Cooked Meat Broth(RCMB)–> Growth of anaerobes (e.g Clostridium)
  6. Alkaline peptone water–> Enrichment media for Vibrio cholerae
  7. Castaneda Medium–> Biphasic medium for isolation of Brucella
  8. Buffered Charcoal Yeast Agar (BCYA)–> Specialized media for isolation of Legionella
  9. Egg Saline Medium–> Preservation of cultures of Gram-negative bacilli
  10. Egg Yolk Agar–> Detection of lipase and lecithinase activity of Clostridium species
  11. Lowenstein-Jensen Medium, Middle brook media–> Selective media for isolation of Mycobacterium tuberculosis
  12. Mueller-Hinton Agar–> Performing antimicrobial susceptibility for bacteria
  13. Wilkins-Chalgren Agar–> Performing antimicrobial susceptibility of anaerobic bacteria
  14. Bile Esculin Agar( contains 40% bile)–> Selective media for Enterococcus species (Black coloration of the medium)
  15. Peptone water–> Basis for indole test, basal media for preparation of carbohydrate fermentation media
  16. Chocolate agar–> Growth of fastidious organisms( e.g. Hemophilus influenzae, Neisseria gonorrhoea, and S. pneumoniae)
  17. Blood agar: Enriched media that support the growth of fastidious organisms. Indicator media to show hemolytic properties:
    • Beta-Hemolytic–>> Staphylococcus aureus
    • Alpha-Hemolytic–>> Streptococcus pneumoniae and S. viridans
    • Non-Hemolytic–>> Enterococcus

About Author

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Sushmita Baniya

Sushmita Baniya is pursuing her Master’s degree in Medical Microbiology from the National College of Science and Technology (NIST), Kathmandu, Nepal. She did her Bachelor’s degree in Microbiology from Birendra Multiple Campus, Chitwan, Nepal. She is interested in Genetics and Molecular Biology.

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