Culture media used in microbiology
Image Source: Sigma-Aldrich
- Nutrient agar(1% to 2%) –> Basal media in microbiology, Supports the growth of all non-fastidious organisms
- MacConkey Agar—> Differential media for Enterobacteriaceae ( i.e. Lactose fermenting and non-lactose fermenting)
- Mannitol Salt Agar–> Selective and indicator media for S. aureus
- Triple Sugar Iron(TSI) medium–> Differentiation of various members of Enterobacteriaceae
- Robertson Cooked Meat Broth(RCMB)–> Growth of anaerobes (e.g Clostridium)
- Alkaline peptone water–> Enrichment media for Vibrio cholerae
- Castaneda Medium–> Biphasic medium for isolation of Brucella
- Buffered Charcoal Yeast Agar (BCYA)–> Specialized media for isolation of Legionella
- Egg Saline Medium–> Preservation of cultures of Gram-negative bacilli
- Egg Yolk Agar–> Detection of lipase and lecithinase activity of Clostridium species
- Lowenstein-Jensen Medium, Middle brook media–> Selective media for isolation of Mycobacterium tuberculosis
- Mueller-Hinton Agar–> Performing antimicrobial susceptibility for bacteria
- Wilkins-Chalgren Agar–> Performing antimicrobial susceptibility of anaerobic bacteria
- Bile Esculin Agar( contains 40% bile)–> Selective media for Enterococcus species (Black coloration of the medium)
- Peptone water–> Basis for indole test, basal media for preparation of carbohydrate fermentation media
- Chocolate agar–> Growth of fastidious organisms( e.g. Hemophilus influenzae, Neisseria gonorrhoea, and S. pneumoniae)
- Blood agar: Enriched media that support the growth of fastidious organisms. Indicator media to show hemolytic properties:
- Beta-Hemolytic–>> Staphylococcus aureus
- Alpha-Hemolytic–>> Streptococcus pneumoniae and S. viridans
- Non-Hemolytic–>> Enterococcus
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