Microbial Genetics

Microbial Genetics

Microbial Genetics Genetics: is the science that studies the inheritance of biological characteristics by living things. This subject, also known as heredity, is a wide-ranging science that examines: The transmission of biological properties (traits) from parents to offspring. The expression and variation of those traits. The structure and function of … Read more

Chemical Factor of Mutation

Chemical Factor of Mutation

Chemical Factor of Mutation What is a mutation? Any changes in the DNA gene sequence in such a way as to alter the genetic message carried by that gene sequence. What is mutagen? Any biological, physical or chemical agent that changes the genetic material, usually DNA of an organism is … Read more

Cell Transformation (normal cells to cancerous cells)

Cell Transformation (normal cells to cancerous cells)

Cell Transformation (normal cells to cancerous cells) Cell transformation is a process in which the addition of certain malignant characteristics in the cell due to alteration in genetic material. induced by chemicals, carcinogens, radiations, viruses. History In 1901 Hugo de Vries first used the term mutation to describe the sudden … Read more

Loop-mediated Isothermal Amplification (LAMP)

Loop-mediated Isothermal Amplification (LAMP)

Introduction ABBREVIATION: Loop-mediated Isothermal AMPlification. Diseases are the primary constrains for the development of any living forms i.e., both plants and animals including humans. Diagnosis is a critical step for effective treatment and control of infectious diseases. PCR (Polymerase Chain Reaction) has revolutionized the earlier detection of infection, tumor, etc., … Read more

RNA Interference (RNAi)

RNA Interference (RNAi)

RNA interference is a natural phenomenon by which an mRNA is silenced thereby inhibiting the protein coded from that particular mRNA. Scientists have been working on this field from 1990 and finally, two scientists named Andrew Fire and Creg C. Mello shared the Nobel Prize in Physiology or Medicine for … Read more

List of disorders due to chromosomal aberration

Disorder due to chromosomal aberration

Image Source: Botany Studies. Chromosomal aberration definition Chromosomal aberration is a condition in which deletion or duplication of a particular chromosome in a set, lead to monosomy, nullisomy, trisomy, tetrasomy. Some disorders in human being due to chromosomal aberrations are as follows: Klinefelter syndrome (XXY) It is due to a … Read more

Vectors- characteristics, classification, features, types

Vectors

Vectors are DNA molecules that are used as a vehicle to carry foreign DNA fragments into other cells where they can replicate and/or express. Among these most commonly used vector is a plasmid. Characteristics of vectors: Self replicating, multiple copies. Replication origin site. Cloning site. Selectable marker gene. Low molecular … Read more

Differences between Real-Time PCR and Rapid Diagnostic Test

Differences between Real-Time PCR and Rapid Diagnostic Test

Rapid Diagnostic Test vs Real-Time PCR (qPCR) There are several diagnostic tests that have been authorized for Emergency Use by the FDA for diagnosis of a wide range of infections. Notably, these diagnostic tests aim at identifying an infected individual from a non-infected person, using different working principles and duration … Read more

COVID-19 diagnosis: Abbott RealTime SARS-CoV-2 assay

Abbott RealTime SARS-CoV-2 assay

Medical Disclaimer: The information presented on the website is only for academic and study purposes and must not be used for the purpose of diagnosis. If you are not feeling well, please consult with your physician or doctor. Introduction We have so far discussed the most current diagnostic tests that … Read more

Vectors for gene cloning: Plasmids and Bacteriophage

Vectors for gene cloning- Plasmids and Bacteriophage

Vectors for gene cloning: Plasmids and Bacteriophage Image Sources: Wikipedia and Vector Stock In molecular biology, a vector refers to a DNA molecule that transfers genetic material into another cell and the genetic material gets to incorporate in the cell genome and expressed. The foreign DNA carried by the vector … Read more

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) It is one of the most important biotechnological tools developed. It refers to a biological technique that helps to produce several copies of DNA outside of any living cell. DNA polymerase is the key enzyme that is present behind the whole process. The enzyme involved in … Read more